Cancer Research Center.
Cancer Research Center.

Cancer Research Center.



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Increased urinary CCL2: Cr ratio in 6 months later the renal allograft loss.

Background: Early non-invasive markers, identification of risk patients renal allograft loss work stratified patients may more monitoring or treatment. Is a CCR2 CCL2 chemokine receptor protein, this is a chemoattractant to monocytes/macrophages, T cells and natural killer cells. We've demonstrates in a multicenter cohort, urine CCL2 in 6 months is an independent predictor of IFTA in 24 months. The goal of this study was to determine the early can predict urine CCL2 graft loss in an independent patient population. Methods: A prospective observational cohort study on the adult kidney transplant Manitoba program (n = 231) from 1997 to 2008. Six months of urine CCL2 measured the ELISA, remove urine creatinine, and long-term transplantation results. Results: Urine CCL2: Cr in 6 months significant related death review graft loss (odds ratio, 2.42; 95% CI, 1.54 to 3.82, P < 0.0001). In multivariate analysis, urine CCL2: Cr in 6 months is still an independent predict death review graft loss (odds ratio, 2.20; 95% CI, 1.18 to 4.10, P = 0.01) after the adjustment before transplantation/DE novo specific antibodies and delayed graft function. Early transplantation (6 months or less) multivariate model the CCL2, the recipient's age, and delayed graft function recovery produced a 0.87 AUC predict death review graft loss. A cut-off value of urine CCL2: Cr 34.8 ng/and a strong positive predictive value is 0.96. Conclusion: The study confirmed in a separate prospective cohort, early urine CCL2 in 6 months is a non-invasive, independent indicate late renal allograft loss.
11.12.12 13:41


Differential regulation of CD4 + T cell adhesion to the brain microvascular endothelial & # 946; The

In multiple sclerosis (MS), circulating lymphocyte through blood & # 8211; Brain barrier (BBB) and accumulation in website antigen challenge. This process depends on the specific lymphocyte and the interaction between the brain microvascular endothelial cell factor, including endothelial cell activation and chemokine existence. Chemokines play a key role, the arrangement of the immune response, agency both chemoattractants and catalyst of white blood cell subset. In the present study, we investigated the influence & # 946; Chemokines, CCL3 CCL2, adhesion and the CD4 + T cell subsets, the human brain microvessel endothelial cells (HBMEC). Chemokine added to the lower cabin bicameral system system at rest or chemotactic cytokines of activation HBMEC tributary, diffusion through the culture substrate and bound to basal surface HBMEC. Low interest rates and adhesion of na # 239; Ve, rest and memory CD4 + T cells rest HBMEC significant adjustment HBMEC treatment with TNF will # 945; And ifn g. Recent CD4 + T cell activation easy to adhere to the rest power. Concentration gradient CCL2 sexual regulation CD4 + T cell activation cell factor treatment but no HBMEC rest. The presence of CCL3 in the house of representatives increase adhesion memory T cells and cytokines HBMEC if treatment. These findings highlight the brain endothelial cell activation of the importance and role, CCL3 CCL2 regulation CD4 + T cell adhesion to BBB endothelial subset, thus specific immune response in MS.
11.12.12 13:39

Macrophages, epithelial cells of the CCL2 help rhinovirus induced high reactivity and inflammation i

Human rhinovirus (HRV) infection that causes disease in asthma attack reduce air, but not in healthy individuals. However, the mechanisms underlying the is still fully understood. We assume that the nuclear drive allergy environment can improve the HRV induced CC chemokine production, which can lead to asthma attacks. Ovalbumin all (OVA) sensitization and challenges vaccinated mice showed a significant increase HRV express lung CCL2 / MCP 1 and aram/MIP - 1 β, CCL7 / MCP 3, CCL19 / MIP - 3 beta and CCL20 / MIP3 α alone than mice with eggs. Inhibiting the CCL2 and neutralizing antibody declined obviously, and HRV induced air inflammation and high reactivity in treatment of mice eggs. Immunohistochemical staining display colocalization CCL2 HRV in epithelial cells and CD68 - positive macrophages and flow cytometry show an increase in the CCL2 (+), CD11b (+) cell lung eggs treatment, HRV infection of mouse. And from a naive mice lung macrophages, macrophages from mice expressed significantly more eggs exposure in response to HRV infection CCL2 in vitro. Pretreatment of mouse and human BEAS - 2 b pulmonary macrophages bronchial epithelial cells and il 4 and il and increase HRV induced the CCL2 expression, and mouse lung macrophages from il 4 receptor knockout mice showed that decreasing response HRV CCL2 expression that come into contact with these individual nucleus cell factor play a role change HRV response. Finally, bronchial alveolar macrophage from children with asthma were described in vitro more CCL2 contact ratio from the asthma patients HRV cell. We come to the conclusion that the epithelial cells of the CCL2 production and macrophage help HRV induced airway hyperresponsiveness and inflammation in a mouse model of allergic aviation disease, may play a role in the HRV induced asthma.
11.12.12 13:38

Thrombin cracking fragment in malignant osteopontin is overexpressed glial tumors, and provides a mo

Osteopontin (OPN), which is highly expression in malignant glioblastoma (GBM), with inflammatory activity, is by thrombin and plasma proteolytic cleavage carboxy peptidase B2 (CPB2) in a highly conservative cleavage site. The OPN (OPN - FL) rise cerebrospinal fluid (CSF) all of the cancer patient samples than patients, the tumor. However thrombin cracking OPN (OPN - R), thrombin/CPB2 - double close to OPN (OPN - L) level obviously improve the GBM and the GBM glioma than systemic cancer and old patients. Close to OPN constitute the ~ 23% and - 31% of total OPN in "the green belt movement" and the GBM cerebrospinal fluid samples respectively. OPN - R are in the green belt movement "organization rise. Thrombin antithrombin level and close to OPN high correlation, but not OPN - FL, this shows that close to OPN fragments lead to thrombin and CPB2 increases in the extracellular space. VEGF and Syria in cerebrospinal fluid and an increased level of "green belt movement" and significant correlation level OPN apart. "Green belt movement" cell line were significantly more adherent to OPN - R and OPN - L than OPN - FL. Adhesion OPN change gene expression, in particular genes involved in the process of cells, cell cycle regulation, death and inflammation. OPN ascension motility u - 87 mg cells and awarded antiapoptotic. Although function variable mother OPN RGD basically abolished these function, OPN (RAA) - r restored important cell binding and signal function, it shows that SVVYGLR mother OPN - r may replace RGD design if the latter become inaccessible. OPN solution to Daniel GBM development allows more cells to binding in the market segment and they have the attributes.
6.12.12 15:24

Enhancement effect function of T cell CD8 from healthy controls and patients with HIV infection is t

Damage blood vessels in the integrity of the trauma and other organizations insult to the inflammation and activation of the coagulation cascade. Serine protease of blood coagulation MeiLian joint the two process. Thrombin proinflammatory function is activated by protease activity receptor 1 (par 1). We found the peripheral blood effector memory CD4 and CD8 T lymphocyte expressed par value 1, expression was increased in patients infected with HIV CD8 T cells. Thrombin enhance the secretion of cytokines in T cell CD8 from healthy control and HIV infected patients. In addition, thrombin induced chemical motility, but not chemotactic sex, CD8 T cells, which can lead to the structural changes, including cell polarization and form a structure rich actin and cpERM (phosphorylation ezrin - radexin - moesin) protein. These findings suggest that thrombin mediated between the coagulation system of crosstalk and adaptive immune system in the web site of vascular injury by increasing the T cell motility and production to promote inflammation cell factor.
6.12.12 15:22

Iatrogenic false aneurysm treatment use ultrasound-guided thrombin injection in during the five year

In this study we show that ultrasonic (US) equipped with injection of thrombin is a safe and effective method to treat iatrogenic false aneurysm as a new treatment in a has 650 beds city community hospitals. We review chart review including patients iatrogenic false aneurysm from January 2004 to June 2010 in a single institution. All patients of pseudo aneurysm patients use we guide injection of thrombin. The research shows that, an overall success rate is 97.1%, the treatment of iatrogenic false aneurysm 33 34 cases. A patient accept open surgery to repair. No death or complications pointed out. The study was a success proves that we guide injection of thrombin is a kind of effective method to treat iatrogenic false aneurysm and can safely for a new treatment model by trained a vascular surgeon. Review of the different techniques include. An algorithm used in the treatment of iatrogenic false aneurysm from this research put forward.
6.12.12 15:20

Reprogramming brings new hop to the treatment of male infertility

September 7, 2012 - / Bio Valley BIOON / Sertoli cells (Sertoli cells) that sex determination in embryonic development and plays a vital role in adulthood spermatogenesis. Has an important role in male fertility, and the suppression of the immune characteristics and neurotrophic properties, so that they will have broad prospects in clinical application. In this study, the researchers of the Massachusetts Institute of Technology, expressed in fibroblasts five transcription factors generated induced embryonic Sertoli-like cells (Sertoli-like cells, ieSCs). The researchers confirmed the specific combination of transcription factors, the role in the process of transformation of fibroblasts to ieSCs, and identified several key steps in the process. Initially, the shift in fibroblasts from the transition of mesenchymal cells to the epithelial cells, and then obtained a aggregation ability, and the formation of the tubular structure, a specific marker expression in the embryo of the Sertoli cells. Sertoli cell-like cells (Sertoli-like) cells can promote the differentiation of nerve cells and neural precursor cells (NPCs) self-renewal, support the survival of the germ cells in vitro culture, and endogenous embryo Sertoli cells and primordial germ cells synergy, facilitate the emergence of the the fetal gonads testicular cord. In conclusion, this study are of great significance to promote the treatment of male infertility, as well as embryonic reproductive and developmental studies.
14.9.12 11:18

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