Observe the relationship tamoxifen resistant breast cancer cells BCAR3 breast cancer anti-estrogen resistance expression and epithelial-mesenchymal transition (EMT) phenomena and migration of breast cancer invasion. Explore the correlation of tamoxifen resistance and breast cancer cell EMT phenomenon. Detection of breast cancer: real-time quantitative PCR and Westernblot epithelioid and mesenchymal-like cell lineBCAR3 breast cancer anti-estrogen resistance 3
expression; Westernblot detect breast cancer MCF27 MCF2BCAR3 and BT2549 mediate EMT transcription factor Twist epithelial marker genes E2 cadherin change of the hormone (E2cadherin) and interstitial marker genes N2 the cadherin (N2cadherin) expression. Transwell invasion assay detection of breast cancer MCF27, MCF2BCAR3 and BT2549 invasion and metastasis. Results: BCAR3 breast cancer anti-estrogen resistance 3
in breast epithelial-like expression in the cell lines was significantly lower than the mesenchymal-like cell line; the MCF27 cell, protein E2cadherin expressed as positive, negative Twist, and N2cadhern expression; tamoxifen Fen-resistant cells MCF2BCAR3 E2cadherin protein is missing, while the Twist and N2cadherin expression positive. BCAR3 breast cancer anti-estrogen resistance 3
overexpression led to the migration and invasion of breast cancer cells MCF27 enhanced. Conclusion: The overexpression BCAR3 enhance breast cancer cell migration and invasion, and tamoxifen treatment become resistant, may BCAR3 induced breast epithelial-mesenchymal transition-related.