Cancer Research Center.
Cancer Research Center.



Cancer Research Center.

  Startseite
  Über...
  Archiv
  Gästebuch
  Kontakt
 

  Abonnieren
 



  Links
   clcf1
   recombinant cd40
   Epitope Mapping
   GAA
   nextgen sequencing
   organic synthesis
   HUman tissue
   CBLC




  Letztes Feedback
   3.10.12 09:15
    lig6oq fnzdmjowthnc, [u
   3.10.12 15:15
    IUCqym    4.06.13 05:30
   
Click For Info
   10.08.13 20:50
       13.08.13 06:56
   
??????????(*'?`*)???????
   20.12.13 08:52
    Ich wollte einfach einen



http://myblog.de/biomichael

Gratis bloggen bei
myblog.de





A experiment about BCAR3 breast cancer anti-estrogen resistance 3

Observe the relationship tamoxifen resistant breast cancer cells BCAR3 breast cancer anti-estrogen resistance expression and epithelial-mesenchymal transition (EMT) phenomena and migration of breast cancer invasion. Explore the correlation of tamoxifen resistance and breast cancer cell EMT phenomenon. Detection of breast cancer: real-time quantitative PCR and Westernblot epithelioid and mesenchymal-like cell lineBCAR3 breast cancer anti-estrogen resistance 3 expression; Westernblot detect breast cancer MCF27 MCF2BCAR3 and BT2549 mediate EMT transcription factor Twist epithelial marker genes E2 cadherin change of the hormone (E2cadherin) and interstitial marker genes N2 the cadherin (N2cadherin) expression. Transwell invasion assay detection of breast cancer MCF27, MCF2BCAR3 and BT2549 invasion and metastasis. Results: BCAR3 breast cancer anti-estrogen resistance 3 in breast epithelial-like expression in the cell lines was significantly lower than the mesenchymal-like cell line; the MCF27 cell, protein E2cadherin expressed as positive, negative Twist, and N2cadhern expression; tamoxifen Fen-resistant cells MCF2BCAR3 E2cadherin protein is missing, while the Twist and N2cadherin expression positive. BCAR3 breast cancer anti-estrogen resistance 3 overexpression led to the migration and invasion of breast cancer cells MCF27 enhanced. Conclusion: The overexpression BCAR3 enhance breast cancer cell migration and invasion, and tamoxifen treatment become resistant, may BCAR3 induced breast epithelial-mesenchymal transition-related.
9.9.12 10:50
 
Letzte Einträge: Iatrogenic false aneurysm treatment use ultrasound-guided thrombin injection in during the five years, Enhancement effect function of T cell CD8 from healthy controls and patients with HIV infection is through the activation of thrombin receptor 1 protease activity., Thrombin cracking fragment in malignant osteopontin is overexpressed glial tumors, and provides a molecular niche and survival advantage, Macrophages, epithelial cells of the CCL2 help rhinovirus induced high reactivity and inflammation in a mouse model of air allergic disease., Differential regulation of CD4 + T cell adhesion to the brain microvascular endothelial & # 946; The CCL2 and CCL3 chemokines, Increased urinary CCL2: Cr ratio in 6 months later the renal allograft loss.


Werbung


bisher 0 Kommentar(e)     TrackBack-URL

Name:
Email:
Website:
E-Mail bei weiteren Kommentaren
Informationen speichern (Cookie)



 Smileys einfügen



Verantwortlich für die Inhalte ist der Autor. Dein kostenloses Blog bei myblog.de! Datenschutzerklärung
Werbung